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fluorescein labeled jacalin 150  (Vector Laboratories)


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    Vector Laboratories fluorescein labeled jacalin 150
    Fluorescein Labeled Jacalin 150, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein labeled jacalin 150/product/Vector Laboratories
    Average 94 stars, based on 46 article reviews
    fluorescein labeled jacalin 150 - by Bioz Stars, 2026-03
    94/100 stars

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    A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with <t>Jacalin</t> (red) and Wheat <t>Germ</t> <t>Agglutinin</t> (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).
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    A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with <t>Jacalin</t> (red) and Wheat <t>Germ</t> <t>Agglutinin</t> (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).
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    A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with <t>Jacalin</t> (red) and Wheat <t>Germ</t> <t>Agglutinin</t> (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).
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    A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with <t>Jacalin</t> (red) and Wheat <t>Germ</t> <t>Agglutinin</t> (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).
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    Image Search Results


    A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with Jacalin (red) and Wheat Germ Agglutinin (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).

    Journal: bioRxiv

    Article Title: Induction of cervical dysfunction associated with preterm birth by IL-1 and dysbiotic microbiome revealed in human endocervix chips

    doi: 10.1101/2025.05.01.651107

    Figure Lengend Snippet: A ) Immunofluorescence microscopic images of histological sections through snap-frozen human endocervix tissue (top) and side view images of a human Endocervix Chip (bottom) showing the mucus layer visualized with Jacalin (red) and Wheat Germ Agglutinin (WGA, green) (bar, 25 μm, tissue; 1 mm, chip). B ) Venn diagram showing the number of unique and common proteins detected in a native pregnancy mucus plug versus mucus cleaved from Endocervix Chips (N=2 human donors) using proteomic analysis. C ) Bar graph showing differential abundance of undecorated, sialylated, sialofucosylated (SF), and sulfated O-glycans in mucus cleaved from Endocervix Chips versus Ectocervix Chips determined using nano LC-MS glycomic profiling. Structures are grouped based on glycan composition and retention time (Hex_HexNAc_NeuAc_Fuc| RT) (N=3-4 chips per condition; Mean ± SD, one-way ANOVA; E2= estradiol, PH= progesterone and estradiol, * p <0.05, ** p <0.01, *** p <0.001).

    Article Snippet: The chips were then perfused with the mucin-binding lectins 4μg/ml Jacalin (Fluorescein; Vector Laboratories, FL-1151-5) and 5μg/ml Wheat Germ Agglutinin (CF568 red fluor, 76221-784) in Hank’s Balanced Salt Solution, HBSS++ for 2 hours at 30μl/hour and subsequently perfused with HBSS++ for additional 2 hours prior to imaging.

    Techniques: Immunofluorescence, Liquid Chromatography with Mass Spectroscopy, Glycoproteomics